Coding
Part:BBa_K1997001:Design
Designed by: Xinyuan Qiu Group: iGEM16_NUDT_CHINA (2016-10-13)
sHRP-C
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 103
Design Notes
Two 10aa linkers were added on both sides of the CDS, sequence were codon optimized for expression in E.coli.
Source
It was custom synthesized. The original sequence was from Armoracia rusticana